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2
CONTENTS
Components of the Kit .......................................................................................3
Quality Control....................................................................................................4
Safety Warnings and Precautions ..............................................................4, 26
Introduction .........................................................................................................5
Materials Not Supplied.......................................................................................8
Protocol ...............................................................................................................9
Supplementary Information.............................................................................11
General guidelines .........................................................................................11
Preparation of template DNA.........................................................................12
Cycle conditions and template quantity.........................................................12
Cycling temperatures.....................................................................................12
Number of cycles and quantity of template ...................................................13
Designing a new sequencing primer .............................................................13
Sequencing PCR products ............................................................................15
Elimination of compressions ..........................................................................15
Reading farther from or closer to the primer .................................................17
Denaturing gel electrophoresis......................................................................18
Troubleshooting ...............................................................................................20
Control DNA Sequence ....................................................................................22
References ........................................................................................................23
Related Products ..............................................................................................24
Contact Information .........................................................................................25