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4
Redivue nucleotides can be stored at 4
°C for up to 1 week after receipt, or
at a constant -20
°C if longer storage is desired. Care must be taken to
prevent evaporation of these small volumes of material. Tightly cap the
vials after use. Store at -20°C between uses if frequency of use is less
than every 1-3 days. If condensation is observed on the walls of the vial or
in the cap, return the liquid to the bottom of the vial and mix well before
use.
QUALITY CONTROL
All kit batches are functionally tested using
33
P labeled terminators and pUC18
double-stranded DNA template as described in this protocol. Release
specifications are based on sequence length, band intensity and sequence
quality. The sequence must be visible up to 300 base pairs on a standardized
gel with less than 24 hours exposure. The sequence must also be free of
background bands strong enough to interfere with sequence interpretation.
SAFETY WARNINGS AND PRECAUTIONS
Warning: For research use only. Not recommended or intended for
diagnosis of disease in humans or animals. Do not use internally or
externally in humans or animals.
Caution: This product is to be used with radioactive material. Please follow the
manufacturer’s instructions relating to the handling, use, storage, and disposal
of such materials.
Warning: Contains formamide. See ‘Material Safety Data Sheet’ on page 26.
All chemicals should be considered as potentially hazardous. We therefore
recommend that this product is handled only by those persons who have been
trained in laboratory techniques and that it is used in accordance with the
principles of good laboratory practice. Wear suitable protective clothing such as
a lab coat, safety glasses, and gloves. Care should be taken to avoid contact
with skin or eyes. In the case of contact with skin or eyes, wash immediately
with water (see ‘Material Safety Data Sheet’ for specific advice).